IRIBARREN PABLO
Congresos y reuniones científicas
Título:
Autophagy down regulates pro-inflammatory mediators in microglial cells and rescues alpha-synuclein induced neuronal cell death
Autor/es:
BUSSI, C; PERALTA RAMOS, JM; ARROYO, DS; IRIBARREN, P
Reunión:
Congreso; Keystone Symposia on Cell Death and Inflammation; 2017
Resumen:
The autophagypathway plays a crucial role in neurodegenerative diseases, although theprecise mechanisms underlying these processes are poorly understood and littleis known about the effects of the autophagic process and its regulation inmicroglial cells (MC).
Theaim of this study was to evaluate the effects of autophagy on the production ofpro-inflammatory mediators by alpha-synuclein (α-syn) stimulated MC, and onneuronal viability in a co-culturemodel. In addition, we studied the autophagy dynamics in MC after α-syn stimulation.
We found that autophagy induction in MC before exogenous α-syn stimulation downregulated IL1β, IL-6,TNF-α and NO production. In addition, italso reduced the phosphorylation of p38 and ERK1/2 MAPKs, which was requiredfor microglial NO production. Besides, we observed in MC/N2A co-cultures thatNO production by MC was necessary for neuronal cell death. Moreover, theinduction of autophagy in MC decreased mRNA iNOS levels and it promotedneuronal cell survival after α-synstimulation