Thyroid tumor cells-fibroblasts crosstalk: role of extracellular vesicles

BRAVO-MIANA, ROCÍO DEL CARMEN; DELLA VEDOVA, ANA BELÉN; DE PAUL, ANA LUCÍA; REMEDI, MARÍA MÓNICA; GUANTAY, MARÍA LAURA; GILARDONI, MÓNICA BEATRIZ; PELLIZAS, CLAUDIA GABRIELA; DONADIO, ANA CAROLINA

Endocrine Connections

Bioscientifica Ltd

Lugar: Bristol; Año: 2020 vol. 9 p. 506 - 506

umor-stroma crosstalk leads to a tumor-promoting microenvironment. In this milieu,extracellular vesicles (EVs) are protagonists in cell-cell communication. Despite thyroidcancer being the most common endocrine malignancy, the contribution of the tumormicroenvironment to thyroid cancer progression is still largely underexplored. We focusedon the role of thyroid tumor cell-fibroblast interaction and EVs as mediators of tumorstromainterplay, in the promotion of thyroid tumor aggressiveness. Thyroid tumor (TPC-1,8505c) or non-tumor thyroid cells (NThyOri) were co-cultured wi th human fibroblasts (Fb).Thyroid cell migration was investigated by the wound-healing assay and actin-networkstaining. Cell-CD147 expression was characterized by flow cytome try. EVs, obtained byultracentrifugation of conditioned media (CMs), were characterized by transmissionelectron-microscopy and CD81 and CD147 expression. Metalloproteinases (MMPs) wereevaluated by zymography in CMs. A migratory phenotype was trig