CpG-DNA promotes Th1 differentiation in aged Balb/c mice

MALETTO, BELKYS; RÓPOLO, ANDREA; MORÓN, GABRIEL; PISTORESI-PALENCIA, MARÍA CRISTINA

FEDERATION AMER SOC EXP BIOL

Año: 2002 vol. 72 p. 447 - 447

We examined whether CpG-DNA couldbe used as adjuvant to induce a T helper cell type-1(Th1) immunity in aged BALB/c mice that showeda Th2 polarization. Bordetella pertussis and completeFreund?s adjuvant (CFA) were used as well.Immunization with ovalbumin (OVA)/CpG-DNAshowed that the immunoglobulin G (IgG)2a/IgG1ratio and OVA-specific T cell response were similarin young and aged mice. OVA/CpG-DNA inducedthe secretion of interferon- (IFN-) and absenceof interleukin (IL)-5. Similar results were found inmice immunized with OVA/CFA. When mice wereimmunized with OVA/B. pertussis, we found thatthe IgG2a/IgG1 ratio and OVA-specific T cell responsewere lower in aged mice and elicited IFN-and IL-5. In vitro CpG-DNA stimulated antigenpresentingcells to display IL-12 and up-regulatethe expression of major histocompatibility complexclass II and B7-2 on B cells as efficiently inaged