IFN gamma priming is involved in the activation of arginase by oligodeoxinucleotides containing CpG motifs in murine macrophages

MIRIAM LISCOVSKY; ROMINA RANOCCHIA; CAROLINA GORLINO; DIEGO ALIGNANI; GABRIEL MORÓN; BELKYS MALETTO; MARÍA C. PISTORESI-PALENCIA

WILEY-BLACKWELL PUBLISHING, INC

Año: 2009 vol. 128 p. 159 - 159

p style="text-align: justify; line-height: 200%;" class="MsoNormal">Recognition of microbial products by macrophages mediates an inflammatory response and plays a critical role in directing the host immune response against infection. In the present work, we show for the first time that synthetic oligonucleotides containing unmethylated cytosine guanine motifs (CpG) in the presence of interferon (IFN)-γ are able to stimulate both arginase and inducible Nitric Oxide Synthase in murine macrophages. Unexpectedly, IFN-γ, a cytokine believed to be an inhibitor of arginase activity, intervenes in the activation of this enzyme. A significant increase in arginase activity was observed upon a short preincubation with IFN-γ (1 hour) and subsequent CpG stimulation. Therefore, a very interesting observation of this study is that the CpG mediated argin