TLR7 triggering with polyU promotes cross-presentation in CD8alpha+ cDCs by enhancing antigen preservation and MHC I-Ag permanence on DC surface

MARÍA INÉS CRESPO; ESTEFANÍA ZACCA; NICOLAS NUÑEZ; ROMINA P. RANOCCHIA; MARIANA MACCIONI; BELKYS A. MALETTO; MARÍA C PISTORESI- PALENCIA.; GABRIEL MORÓN

AMER ASSOC IMMUNOLOGISTS

Lugar: Bethesda; Año: 2013 vol. 190 p. 948 - 948

!--[if gte mso 9]> Single stranded RNA (ssRNA) can interact with DCs through binding to Toll-like receptor (TLR) 7, inducing secretion of pro-inflammatory cytokines and type I IFN. Triggering TLR7 enhances cross-priming of CD8+ T cells, which requires cross-presentation of exogenous antigen (Ag) to dendritic cells (DCs). However, how TLR triggering can affect Ag cross-presentation is still not clear. Using ovalbumin (OVA) as an antigen model, we observed that stimulation of TLR7 in DCs by polyuridylic acid (polyU), a synthetic ssRNA analog, generates a strong specific cytotoxic response in C57BL/6 mice. PolyU stimulate