CpG-ODN formulated with a nanostructure as adjuvant for anticrotalic serum production. Studies in mice

LUCIANO S. FUSCO; MARÍA M. PASCUAL; DAVID HERNANDEZ; MARÍA FERNANDA S. VALLECITO; MARÍA B. ARRIETA; MORON, GABRIEL; SANTIAGO PALMA; BELKYS MALETO; LAURA C. LEIVA

TOXICON

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2022 vol. 215 p. 28 - 28

0041-0101

ntivenom is the only safe and effective treatment to neutralize snake venom. Specificanti-venom used to treat snake bite is usually obtained from horses afterhyperimmunization with crude snake venom in combination with Freund’s Adjuvant.Freund´s complete and incomplete adjuvant can cause severe local and systemicacute and chronic inflammation, its potentially severe inflammatory effects have ledmany researchers to seek alternative immunological adjuvants. CpG-ODN formulatedin a 6-O-ascorbyl palmitate nanostructure (Coa-ASC16) was more efficient as adjuvantthan CpG ODN alone using ovalbumin (OVA) as an antigen model. Particularly,immunization of mice with OVA/CpG-ODN/Coa-ASC16 resulted in high OVA specificantibody titers and IFN-γ and IL-17 secretion compared to immunization withOVA/CpG-ODN. First of all, we estimated the effect of Coa-ASC16 nanostructurepreparation on venom activity. Additionally, in order to evaluate the immune responseinduced by this adjuvant strategy using Cro