High-dimensional analysis of sixteen SARS-CoV-2 vaccine combinations reveals lymphocyte signatures correlating with immunogenicity

NICOLAS NUÑEZ; JONAS SCHMID; LAURA POWER; CHIARA ALBERTI; SINDUYA KRISHNARAJAH; STEFANIE KREUTMAIR; SUSANNE UNGER; SEBASTIÁN BLANCO; BRENDA KONIGHEIM; CONSTANZA MARIN; LUISINA ONOFRIO; JENNY CHRISTINE KIENZLER; SARA DA COSTA PEREIRA; FLORIAN INGELFINGER; GRUPO INMUNOCOVIDCBA; MARINA E. PASINOVICH; JUAN M CASTELLI; CARLA VIZZOTTI; MAXIMILIAN SCHAEFER; JUAN VILLAR-VESGA; SARAH MUNDT; CARLA HELENA MERTEN; AAKRITI SETHI; TOBIAS WERTHEIMER; MIRJAM LUTZ; DANUSIA VANOAICA; CLAUDIA SOTOMAYOR; ADRIANA GRUPPI; CHRISTIAN MÜNZ; DIEGO CARDOZO; GABRIELA BARBÁS; LAURA LOPEZ; PAULA CARREÑO; GONZALO CASTRO; ELIAS RABOY; SANDRA GALLEGO*; GABRIEL MORÓN*; LAURA CERVI*; EVA V ACOSTA RODRIGUEZ*; BELKYS A. MALETTO*; MACCIONI, MARIANA*; BURKHARD BECHER*

NATURE IMMUNOLOGY (PRINT)

NATURE PUBLISHING GROUP

Lugar: Londres; Año: 2023

1529-2908

span style="font-size: 11pt; line-height: 115%; font-family: "Arial", sans-serif;" lang="en">The COVID-19 pandemicled to the development of a broad range of vaccines against SARS-CoV-2. Thisoffers a unique opportunity to study the human immune response to differenttypes of vaccines and their heterologous combinations. Here, we show asingle-cohort characterisation of changes to the humoral and cellular immunecompartments following five different COVID-19 vaccines spanning three technologies(adenoviral, mRNA and inactivated vaccines). These vaccines were administeredin a combinatorial fashion, resulting in sixteen different homologous andheterologous regimens. Single-cell analysis of 754 samples revealed thatpriming with the inactivated-virus vaccine BBIBP-CorV induced lessclass-switching among spike-binding memory B cells and led to a higherfrequency of CD4-CD8- T cell clusters as well as thehighest antigen-specific T cell responses. In contrast, BBIBP-C