Human hydatid cyst fluid-induced therapeutic anti-cancer immune responses via NK1.1+ cell activation in mice

BERRIEL, EDGARDO; FREIRE, TERESA; CHIALE, CAROLINA; RODRÍGUEZ, ERNESTO; MORÓN, GABRIEL; FERNÁNDEZ-GRAÑA, GABRIEL; CRISPO, MARTINA; BEROIS, NORA; OSINAGA, EDUARDO

CANCER IMMUNOLOGY IMMUNOTHERAPY

SPRINGER

Año: 2021 vol. 70 p. 3617 - 3617

0340-7004

chinococcus granulosus is a cestode parasite which causes cystic echinococcosis disease. Previously we observed that vaccination with E. granulosus antigens from human hydatid cyst fluid (HCF) significantly inhibits colon cancer growth. In the present work, we evaluate the anti-tumor immune response induced by human HCF against LL/2 lung cancer in mice. HCF vaccination protected from tumor growth, both in prophylactic and therapeutic settings, and significantly increased mouse survival compared to control mice. Considering that tumor-associated carbohydrate antigens are expressed in E. granulosus, we oxidized terminal carbohydrates in HCF with sodium periodate. This treatment abrogates the anti-tumor activity induced by HCF vaccination. We found that HCF vaccination-induced IgG antibodies that recognize LL/2 tumor cells by flow cytometry. An antigen-specific immune response is induced with HCF vaccination in the tumor-draining lymph nodes and spleen characterized by the production of