MORÓN VÍCTOR GABRIEL
Congresos y reuniones científicas
Título:
Enhancement of immunological activity of CpG-ODN vehiculized in lyotropic liquid crystal carriers
Autor/es:
BELKYS MALETTO; DANIEL ALLEMANDI; GABRIEL MORÓN; MARÍA C. PISTORESI-PALENCIA; SANTIAGO PALMA
Lugar:
Tandil, Buenos Aires
Reunión:
Congreso; XL Reunión de la Sociedad Argentina de Farmacología Experimental; 2008
Institución organizadora:
Sociedad Argentina de Farmacología Experimental
Resumen:
CpG-ODN (oligonucleotides containing unmethylated CpG in
particular base context) is a new adjuvant for vaccines that
unfortunately has shown reduced bioavailability owed mainly to
lack of chemical stability. In this work, we evaluated the
adjuvant capacity of CpG-ODN incorporated into lamellar
mesophases of lyotropic liquid crystals (coagels). For that,
BALB/c mices were immunized (s.c.) three times during fifteen
days (schedule: 0, 7 and 15) with a protein antigen (OVA, 60
ug/mice/dose) plus CpG-ODN (50ug/mice/dose) or OVA/CpGODN/
coagel. On day 21, we measured specific antibodies in
plasma and specific cytokines in cell culture supernatants
(ELISA). The measured titles (log10) were:
IgG IgG1 IgG2a
OVA/CpG-ODN 3,9±0,0 3,4±0,1 2,7±0,2
OVA/CpG-ODN/coagel 5,1±0,0 4,4±0,1 3,8±0,2
The levels of IFN- were higher in animals immunized with
OVA/CpG-ODN/coagel than in those immunized with
OVA/CpG-ODN (p<0,005). These results indicate that CpGODN
intensified the specific immune response when
administered in coagels.
particular base context) is a new adjuvant for vaccines that
unfortunately has shown reduced bioavailability owed mainly to
lack of chemical stability. In this work, we evaluated the
adjuvant capacity of CpG-ODN incorporated into lamellar
mesophases of lyotropic liquid crystals (coagels). For that,
BALB/c mices were immunized (s.c.) three times during fifteen
days (schedule: 0, 7 and 15) with a protein antigen (OVA, 60
ug/mice/dose) plus CpG-ODN (50ug/mice/dose) or OVA/CpGODN/
coagel. On day 21, we measured specific antibodies in
plasma and specific cytokines in cell culture supernatants
(ELISA). The measured titles (log10) were:
IgG IgG1 IgG2a
OVA/CpG-ODN 3,9±0,0 3,4±0,1 2,7±0,2
OVA/CpG-ODN/coagel 5,1±0,0 4,4±0,1 3,8±0,2
The levels of IFN- were higher in animals immunized with
OVA/CpG-ODN/coagel than in those immunized with
OVA/CpG-ODN (p<0,005). These results indicate that CpGODN
intensified the specific immune response when
administered in coagels.
