MORÓN VÍCTOR GABRIEL
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Título:
RECRUITMENT OF IMMUNOCOMPLEX-ACTIVATED NEUTROPHILS MODULATE T CELL RESPONSE IN LYMPH NODES
Autor/es:
SOFÍA CASTELL; FLORENCIA HARMAN; MORON VG; BELKYS A. MALETTO; MARÍA C PISTORESI- PALENCIA
Lugar:
Mar del Plata
Reunión:
Congreso; LXIV Reunión de la Sociedad Argentina de Inmunología; 2016
Institución organizadora:
Sociedad Argentina de Inmunolog
Resumen:

Previouslywe demonstrated that immunocomplexes (IC) generated by injecting OVA in thefootpad of immunized mice that have anti-OVA antibodies, induce neutrophils-OVA+ migration to draining popliteal lymph nodes (D-poLNs). In the presentstudy we evalu- ate the influence of IC-activated neutrophils on T-cellresponse in lymph nodes. C57BL/6 mice were immunized with OVA emulsified inFreund´s complete adjuvant and 15 days later boosted with OVA emulsified inFreund´s incomplete adjuvant. Ten days after last immunization they wereinjected with OVA-FITC in the footpad and D-poLNs were obtained at differenttimes (between 3 and 48 h); saline solution was injected on footpadcorresponding to control non-draining popliteal lymph nodes (ND-poLNs). Thedif- ferent cell populations in the lymph nodes were characterized by flowcytometry. Three hours after footpad injection, an increased number of OVA-FITC+ Ly6Ghi neutrophils was observed in D-poLNscompared to ND-poLNs (p<0.05). The highest levels of OVA-FITC+ neutrophils were detected 6 h after OVA injection (p<0.001), at 12 hthe number of neutrophils decreased to basal levels. However, total number ofD-poLN cells increase after 24 h of OVA injec- tion (p<0.01). At24 h weobserved an increase of dendritc cells (CD11c+) inD-poLNs compared to ND-poLNs (p<0.01). At 48 h we observed an increase ofCD4 T cells (p<0.01), particularly effector CD4 T-cells (CD44+ CD62L-) (p<0.01) with respect to ND-poLNs.Besides, we observed higher percentage of Ki67+ CD4T-cells in immunized mice 24 h after OVA injection (p<0.001), indicatingthat CD4 T-cells have higher levels of proliferation after recruitment ofIC-activated neutrophils. These results suggest that neutrophils recruitment indraining popliteal lymph nodes could be modulating the T-cell response.

 

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