MORÓN VÍCTOR GABRIEL
Congresos y reuniones científicas
Título:
124. A NANOSTRUCTURE OF ASCORBYL PALMITATE USED AS VACCINE PLATFORM IMPROVE ANTIGEN-SPECIFIC MEMORY RESPONSE AND RETAINS THE ANTIGEN AT THE INJECTION SITE
Autor/es:
FEDERICO DANIEL RUIZ MORENO; CONSTANZA MARIN; NICOLAS DANIEL DHO; MERCEDES PASCUAL; DANIEL ALLEMANDI; SANTIAGO PALMA; MARÍA C PISTORESI- PALENCIA; G MORON; BELKYS A. MALETTO
Lugar:
Buenos Aires
Reunión:
Congreso; LXVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2020
Institución organizadora:
SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI)
Resumen:
Previously, we demonstrated that the nanoformulation of OVA
and CpG-ODN with a nanostructure formed by self-assembly of
6-O-ascorbyl palmitate (Coa-ASC16) elicited immune response
superior to those induced by the soluble counterpart. Here, we
studied the effects of various formulations of vaccine components
on antigen-specific memory response and on antigen persistence
at injection site. Mice were subcutaneously immunized with a single-dose of OVA and CpG-ODN nanoformulated with Coa-ASC16
(OCC), with OVA and CpG-ODN in solution (OC) prepared to room
temperature (RT), with OVA and CpG-ODN solution heated and then
cooled down to RT (OCø), with OVA solution heated and then cooled
down to RT plus CpG-ODN solution at RT (Oø/C), with OVA solution
at RT plus CpG-ODN solution heated and then cooled down to RT
(O/Cø). Heating and cooling processes recreated the conditions of
the nanoformulation preparation. On the 160th day of post-immunization
(pi), mice were intraperitoneally challenged with OVA/CpGODN
and sacrificed 7 days later. OVA-anti IgG titers (ELISA) and
splenocytes by flow cytometry were evaluated. When antigens at
the injection site were measured, the formulations were prepared
using fluorescent-dye labeled OVA and in vivo scanning of mice
was performed on Odyssey®CLx. OCC induced IgG titers higher
than OCø and Oø/C at 145 day pi (p<0.001). Post challenged (day
167), OCC IgG titers were comparable to OCø group and higher to
Oø/C group (p<0.01). O/Cø failed to generate OVA-specific IgG. At
167 days pi, OCC generated higher OVA-specific (CD3-F480- CD19+
IgM- IgD- IgG+ GL7- CD38+ OVA+) memory and germinal center (CD3-
F480- CD19+ IgM- IgD- IgG+ GL7+ CD38- OVA+) B cells (p<0.05) than
the other formulations. In OCC group, the OVA fluorescence signal
in the injection site was higher than in the other groups since 0.3
(p<0.0001) until 12 days pi (p<0.01). Our results show that the nanostructure
improve the memory response and induce antigen depot
at the injection site.
and CpG-ODN with a nanostructure formed by self-assembly of
6-O-ascorbyl palmitate (Coa-ASC16) elicited immune response
superior to those induced by the soluble counterpart. Here, we
studied the effects of various formulations of vaccine components
on antigen-specific memory response and on antigen persistence
at injection site. Mice were subcutaneously immunized with a single-dose of OVA and CpG-ODN nanoformulated with Coa-ASC16
(OCC), with OVA and CpG-ODN in solution (OC) prepared to room
temperature (RT), with OVA and CpG-ODN solution heated and then
cooled down to RT (OCø), with OVA solution heated and then cooled
down to RT plus CpG-ODN solution at RT (Oø/C), with OVA solution
at RT plus CpG-ODN solution heated and then cooled down to RT
(O/Cø). Heating and cooling processes recreated the conditions of
the nanoformulation preparation. On the 160th day of post-immunization
(pi), mice were intraperitoneally challenged with OVA/CpGODN
and sacrificed 7 days later. OVA-anti IgG titers (ELISA) and
splenocytes by flow cytometry were evaluated. When antigens at
the injection site were measured, the formulations were prepared
using fluorescent-dye labeled OVA and in vivo scanning of mice
was performed on Odyssey®CLx. OCC induced IgG titers higher
than OCø and Oø/C at 145 day pi (p<0.001). Post challenged (day
167), OCC IgG titers were comparable to OCø group and higher to
Oø/C group (p<0.01). O/Cø failed to generate OVA-specific IgG. At
167 days pi, OCC generated higher OVA-specific (CD3-F480- CD19+
IgM- IgD- IgG+ GL7- CD38+ OVA+) memory and germinal center (CD3-
F480- CD19+ IgM- IgD- IgG+ GL7+ CD38- OVA+) B cells (p<0.05) than
the other formulations. In OCC group, the OVA fluorescence signal
in the injection site was higher than in the other groups since 0.3
(p<0.0001) until 12 days pi (p<0.01). Our results show that the nanostructure
improve the memory response and induce antigen depot
at the injection site.
