MORÓN VÍCTOR GABRIEL
Congresos y reuniones científicas
Título:
DYNAMICS OF SOLUBLE IMMUNE MEDIATORS IN COVID-19 PATIENTS FROM AN ARGENTINEAN COHORT WITH MODERATE AND SEVERE SYMPTOMS
Autor/es:
LAURA ALMADA; OTROS (VER EN RESUMEN); G MORON; GRUPO INMUNOCOVIDCBA
Lugar:
virtual
Reunión:
Congreso; LXIX REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2021
Institución organizadora:
SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI)
Resumen:
Laura Almada*, Sofia C Angiolini*, Nicolás Dho N*, Jeremias
Dutto J*, Yamila Gazzoni Y*, Clarisa Manzone C*, Constanza
Marín *, Nicolás E Ponce, Pablo Iribarren, Fabio Cerbán,
Gabriel Morón, Carolina Amezcua Vesely, Yamile Ana, Laura
Cervi, Laura Chiapello, Laura Fozzatti L, Paula Icely, Mariana
Maccioni, Cristian Mena, Carolina Montes, Cristina Motrán ,
Cecilia Rodríguez Galán, Cinthia Stempin, María Estefania
Viano, M Bertone#, Claudio Abiega#, Daiana Escudero#,
Adrian Kahn#, Juan Pablo Caeiro#, Daniela Arroyo, Belkys
Maletto , Eva Acosta Rodríguez, Adriana Gruppi, Claudia E
Sotomayor. Grupo ImmunoCovidCBA.
*All these authors contributed equally.
Departamento de Bioquímica Clínica, CIBICI-CONICET, Facultad
de Ciencias Químicas, Universidad Nacional de Córdoba.
#Hospital Privado Universitario de Córdoba, IUCBC.
The cytokine storm, a form of systemic inflammatory response syndrome,
is one of the most dreadful complications that can occur
during COVID-19. In this work, we aimed at studying the occurrence
of a cytokine storm in a cohort of COVID-19 patients (Cpts) from Córdoba
(Argentine). During first wave, we collected sera from individuals
with RT-PCR+ for SARS-CoV2 hospitalized in Hospital Privado
with moderate (MOD) and severe (SEV) disease (n=62, aged 21-80
years) as well as healthy controls (HC n=24, age matched), to determine
the concentrations of IL-1b, IL-6, IL-8, IL-10, IL-12, IL-28A/B,
IL-29, TNF, IP-10, IFNa2, IFNb, IFNg and GM-CSF by LEGENDplexTM.
Males represented 74% of MOD and 67% of SEV Cpts. Hypertension
(HT, 48%), obesity (31%), dyslipidemia (DL, 24%), and
diabetes (24%) were the most frequent comorbidities. All cytokines,
except IL-28A/B, were significantly increased in total Cpts in comparison
with HC (p<0.01). Elevated levels of IL-6 and CRP (p<0.01)
between 4-7 day after hospitalization were found in all Cpts who
died, but the cytokine profiles were different in deceased SEV than
in MOD Cpts. Mortality in SEV group was associated with high levels
of IL-6 (p<0.001), GM-CSF (p<0.01), IL-8, CRP, leukocytosis and
decreased platelets (p<0.0001 for all). Comorbidities were linked to
particular patterns of immune mediators on admission but not afterwards
during infection, with HT Cpts exhibiting increased IP-10 levels
but DL Cpts showing lower concentration of IL-1b, GM-CSF and IL-10 compared to no-HT and no-DL Cpts, respectively (p<0.05).
The frequency of Cpts who required O2 support was higher in HT
(84%) vs DL (67%). Although our data have similarities with those
in international reports, the complete profiling of different parameters
(cytokine/chemokines, risk factors, epidemiological and clinical
characteristics) in the local cases add value by identifying particularities
that may be relevant for the management and prognosis during
SARS-CoV2 infection.
Dutto J*, Yamila Gazzoni Y*, Clarisa Manzone C*, Constanza
Marín *, Nicolás E Ponce, Pablo Iribarren, Fabio Cerbán,
Gabriel Morón, Carolina Amezcua Vesely, Yamile Ana, Laura
Cervi, Laura Chiapello, Laura Fozzatti L, Paula Icely, Mariana
Maccioni, Cristian Mena, Carolina Montes, Cristina Motrán ,
Cecilia Rodríguez Galán, Cinthia Stempin, María Estefania
Viano, M Bertone#, Claudio Abiega#, Daiana Escudero#,
Adrian Kahn#, Juan Pablo Caeiro#, Daniela Arroyo, Belkys
Maletto , Eva Acosta Rodríguez, Adriana Gruppi, Claudia E
Sotomayor. Grupo ImmunoCovidCBA.
*All these authors contributed equally.
Departamento de Bioquímica Clínica, CIBICI-CONICET, Facultad
de Ciencias Químicas, Universidad Nacional de Córdoba.
#Hospital Privado Universitario de Córdoba, IUCBC.
The cytokine storm, a form of systemic inflammatory response syndrome,
is one of the most dreadful complications that can occur
during COVID-19. In this work, we aimed at studying the occurrence
of a cytokine storm in a cohort of COVID-19 patients (Cpts) from Córdoba
(Argentine). During first wave, we collected sera from individuals
with RT-PCR+ for SARS-CoV2 hospitalized in Hospital Privado
with moderate (MOD) and severe (SEV) disease (n=62, aged 21-80
years) as well as healthy controls (HC n=24, age matched), to determine
the concentrations of IL-1b, IL-6, IL-8, IL-10, IL-12, IL-28A/B,
IL-29, TNF, IP-10, IFNa2, IFNb, IFNg and GM-CSF by LEGENDplexTM.
Males represented 74% of MOD and 67% of SEV Cpts. Hypertension
(HT, 48%), obesity (31%), dyslipidemia (DL, 24%), and
diabetes (24%) were the most frequent comorbidities. All cytokines,
except IL-28A/B, were significantly increased in total Cpts in comparison
with HC (p<0.01). Elevated levels of IL-6 and CRP (p<0.01)
between 4-7 day after hospitalization were found in all Cpts who
died, but the cytokine profiles were different in deceased SEV than
in MOD Cpts. Mortality in SEV group was associated with high levels
of IL-6 (p<0.001), GM-CSF (p<0.01), IL-8, CRP, leukocytosis and
decreased platelets (p<0.0001 for all). Comorbidities were linked to
particular patterns of immune mediators on admission but not afterwards
during infection, with HT Cpts exhibiting increased IP-10 levels
but DL Cpts showing lower concentration of IL-1b, GM-CSF and IL-10 compared to no-HT and no-DL Cpts, respectively (p<0.05).
The frequency of Cpts who required O2 support was higher in HT
(84%) vs DL (67%). Although our data have similarities with those
in international reports, the complete profiling of different parameters
(cytokine/chemokines, risk factors, epidemiological and clinical
characteristics) in the local cases add value by identifying particularities
that may be relevant for the management and prognosis during
SARS-CoV2 infection.
