MORÓN VÍCTOR GABRIEL
Congresos y reuniones científicas
Título:
PHENOTYPIC AND FUNCTIONAL CHARACTERIZATION OF PERIPHERAL T CELL POPULATIONS FROM COVID-19 PATIENTS HOSPITALIZED IN HOSPITAL PRIVADO UNIVERSITARIO CÓRDOBA- ARGENTINA.
Autor/es:
LUISINA ONOFRIO; OTROS (VER EN RESUMEN); G MORON; GRUPO IMMUNOCOVIDCBA
Lugar:
virtual
Reunión:
Congreso; LXIX REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2021
Institución organizadora:
SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI)
Resumen:
Luisina Onofrio*, Jeremías Dutto*, Sabrina Bossio*, Ruth E. Baigorri*, María Belén Brugo*, Laura Almada**, Constanza
Marín**, Federico Ruiz Moreno**, Ximena Volpini**, Mariel
Almeida, Carolina Olivera, Nicolás Ponce, Juan Nahuel Quiroz,
Silene Silvera Ruiz, Lucia Bofelli, Eva Acosta Rodríguez,
Carolina Amezcua Vesely, Daniela Arroyo, Fabio Cerbán,
Laura Cervi, Laura Fozzatti, Paula Icely, Pablo Iribarren,
Belkys Maletto, Gabriel Morón, Cecilia Rodríguez Galán,
Cinthia Stempin, Claudio Abiega#, Daina Escudero#, Adrian
Kahn#, Juan Pablo Caeiro#, Mariana Maccioni, Cristina
Motrán, Laura Chiapello, Carolina Montes, Adriana Gruppi,
Claudia E Sotomayor. Grupo ImmunoCovidCBA
*All these authors contributed equally. **All these authors
contributed equally
Departamento de Bioquímica Clínica, CIBICI-CONICET, Facultad
de Ciencias Químicas, Universidad Nacional de Córdoba.
#Hospital Privado Universitario de Córdoba, IUCBC.
SARS-CoV-2 infection results in asymptomatic, mild or severe
disease. T cells could contribute to these different outcomes, but
it remains unclear whether T cell response is dysfunctional or excessive.
Here, we evaluated the phenotypic and functional features
of circulating T cells from a cohort of 40 COVID-19 patients (Cpts)
with moderate (MOD) and severe (SEV) clinical disease (aged 21-
80 years) and 14 aged matched healthy controls (HC) by FACS. All
Cpts exhibited a reduced frequency of CD3+T and Tregs cells compare
to HC (p< 0.05). When exhaustion was evaluated, SEV Cpts
showed higher % of PD-1+ and CD39+ in T conv cells (p<0.05),
whereas no differences were found in BTLA or TIGIT expression.
Even though, no differences in cytokine production (INF-γ, IL-2 and
TNF) were observed, T conv cells from SEV Cpts showed a higher
% of GZMB+ and CD107+ cells than MOD Cpts or HC (p<0.05). Circulating
CD8+ T cells express different levels of CD8, where CD8lo
cells represent highly activated cytotoxic T cells. COVID-19 patients
presented a higher % of CD8lo T cells than controls and this increment
was even more pronounced in SEV Cpts (p=0.04, MD vs
HD; p=0.0012, SD vs HD). CD8lo T cells exhibited impaired cytokine
production and CD107a expression compared to CD8hi T cells, although
GZMB levels were similar among both CD8+ subsets. CD8lo
population from HC showed higher % of naïve T cells than effector
memory (EM)(p=0.04) or EMRA (p=0.008) subsets, but this distribution
was not seen in MOD or SEV Cpts. Indeed, MOD or SEV Cpts
showed higher % of EM cells than HC.
Conclusion: the disease severity impacts on the phenotype and
functional features of CD4+ and CD8+ T cells, with a pronounced
increment in the % of CD8lo T cells as the disease worsens. These
cells appear to have dysfunctional phenotype with an impairment
of effector cytokines production but maintaining cytotoxic potential.
The CD8hi/CD8lo ratio might be a useful parameter to predict the
disease outcome.
Marín**, Federico Ruiz Moreno**, Ximena Volpini**, Mariel
Almeida, Carolina Olivera, Nicolás Ponce, Juan Nahuel Quiroz,
Silene Silvera Ruiz, Lucia Bofelli, Eva Acosta Rodríguez,
Carolina Amezcua Vesely, Daniela Arroyo, Fabio Cerbán,
Laura Cervi, Laura Fozzatti, Paula Icely, Pablo Iribarren,
Belkys Maletto, Gabriel Morón, Cecilia Rodríguez Galán,
Cinthia Stempin, Claudio Abiega#, Daina Escudero#, Adrian
Kahn#, Juan Pablo Caeiro#, Mariana Maccioni, Cristina
Motrán, Laura Chiapello, Carolina Montes, Adriana Gruppi,
Claudia E Sotomayor. Grupo ImmunoCovidCBA
*All these authors contributed equally. **All these authors
contributed equally
Departamento de Bioquímica Clínica, CIBICI-CONICET, Facultad
de Ciencias Químicas, Universidad Nacional de Córdoba.
#Hospital Privado Universitario de Córdoba, IUCBC.
SARS-CoV-2 infection results in asymptomatic, mild or severe
disease. T cells could contribute to these different outcomes, but
it remains unclear whether T cell response is dysfunctional or excessive.
Here, we evaluated the phenotypic and functional features
of circulating T cells from a cohort of 40 COVID-19 patients (Cpts)
with moderate (MOD) and severe (SEV) clinical disease (aged 21-
80 years) and 14 aged matched healthy controls (HC) by FACS. All
Cpts exhibited a reduced frequency of CD3+T and Tregs cells compare
to HC (p< 0.05). When exhaustion was evaluated, SEV Cpts
showed higher % of PD-1+ and CD39+ in T conv cells (p<0.05),
whereas no differences were found in BTLA or TIGIT expression.
Even though, no differences in cytokine production (INF-γ, IL-2 and
TNF) were observed, T conv cells from SEV Cpts showed a higher
% of GZMB+ and CD107+ cells than MOD Cpts or HC (p<0.05). Circulating
CD8+ T cells express different levels of CD8, where CD8lo
cells represent highly activated cytotoxic T cells. COVID-19 patients
presented a higher % of CD8lo T cells than controls and this increment
was even more pronounced in SEV Cpts (p=0.04, MD vs
HD; p=0.0012, SD vs HD). CD8lo T cells exhibited impaired cytokine
production and CD107a expression compared to CD8hi T cells, although
GZMB levels were similar among both CD8+ subsets. CD8lo
population from HC showed higher % of naïve T cells than effector
memory (EM)(p=0.04) or EMRA (p=0.008) subsets, but this distribution
was not seen in MOD or SEV Cpts. Indeed, MOD or SEV Cpts
showed higher % of EM cells than HC.
Conclusion: the disease severity impacts on the phenotype and
functional features of CD4+ and CD8+ T cells, with a pronounced
increment in the % of CD8lo T cells as the disease worsens. These
cells appear to have dysfunctional phenotype with an impairment
of effector cytokines production but maintaining cytotoxic potential.
The CD8hi/CD8lo ratio might be a useful parameter to predict the
disease outcome.
