Unraveling the Spatial Distribution of Immunoglobulins, Enzymes, and Polyelectrolytes within Layer-by-Layer Self-Assembled Multilayers. Ellipsometric studies.

NANCY F. FERREYRA; ERICA S. FORZANI; MANUEL LOPEZ TEIJELO; LILIANE COCHE-GUERENTE; PIERRE LABBE

American Chemical Society

Año: 2006 vol. 22 p. 8931 - 8931

font size="1" face="Times New Roman"> The ellipsometric characterization of a layer-by-layer electrostatically self-assembled multilayer of polyphenol oxidase and alkaline phosphatase with the polycation poly(dimethyldiallylammonium chloride) built on an immunologic layer formed by immunoglobulin G (IgG) and glucose oxidase-conjugated anti-IgG (IgG-GOD) on glassy carbon is reported. The step-by-step evolution of the ã-¢ ellipsometric angles was followed during film growth. Two optical models, named the three-layer film model and reorganization film model, were employed and found suitable for ellipsometric data interpretation. A comparative analysis of film optical properties, film thi