Synthesis and characterization of resorbable films for odontologic use with controlled drug release properties

CAMACHO NAHUEL MATIAS; PALMA SANTIAGO DANIEL; GONZÁLEZ AGUSTÍN

Rosario

Congreso; 4ta Reunión Internacional de Ciencias Farmacéuticas; 2016

Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario

Resorbable films are often used in odontologic area for guided tissue regeneration and wound protect. Actually, in periodontal surgery profilaxis, dentists use an antibiotic topical solution on wound. This methodology is poorly efficient and attemps against the rational use the drug. For this reason, the controlled drug release from these films represents a required and necessary development. In this work, Chistosan (Ch) and collagen/chitosan (Cg/Ch) films were synthesized by ?casting? and commercial porcine pericardium films (Cgp) were modified. Those films were chemically crosslinked and physiochemical and mechanical characterized. It was determined that the swelling capacity of Cgp´s was higher respect films prepared by casting and it markedly decreased with the crosslinking degree. The same behavior was observed in total soluble matter in water determination. By performing tensile tests was observed that cross-linking results in more fragile and brittle films, since lower tensile strengths and elongation at break were observed. Once the different films were characterized we proceeded to load them with gentamicin using three different methodologies. In-vitro drug release profiles were determined in saliva simulated solutions where a sustained release was observed. It was measured that Cgp films required about 7 days to release all contained drug. Additionally in all cases the influence of the crosslinking degree was observed in the release profiles. Furthermore, the activity of the incorporated drug was demonstrated by in-vitro bacteria inhibiting growth tests (Staphylococcus aureus).