Resumen:
he customization of the properties of layered double hydroxides nanoparticles (LDH-NPs) is essential to opti- mize their design innovative systemsfor biomedical applications. Response surfacemethodologies (RSMs), a col- lection ofmathematical and statistical techniques that allowsmultivariate optimization of processes, are a useful tool for LDH-NPs customization. In this work, RSMswere used to study the synthesis parameters governing the size and composition of MgAl LDH-NPs(Mg/Al=3) labeledwith fluorescein isothiocyanate (FITC2−) and pre-pare customized samples for biological assays. The influence of [Al3+], [OH−]/[Mg2++Al3+], 2[FITC2−]/[Al3+] and time of the hydrothermal treatment (tHT) was determined by a Box-Behnken experimental design (BBD). The experimental responses were hydrodynamic diameter (dH), polidispersity index (PI) and FITC2− content (%FITC), and their dependencewith the synthesis variableswas described by polynomial model. The model cor- related