Resumen:
seudomonas strains producing antimicrobial secondary metabolites play an important role in the biocontrol
of phytopathogenic fungi. In this study, native Pseudomonas spp. isolates were obtained from the
rhizosphere, endorhizosphere and bulk soil of maize fields in Córdoba (Argentina) during both the vegetative
and reproductive stages of plant growth. However, the diversity based on repetitive-element PCR
(rep-PCR) and amplified ribosomal DNA restriction analysis (ARDRA) fingerprinting was not associated
with the stage of plant growth.
Moreover, the antagonistic activity of the native isolates against phytopathogenic fungi was evaluated
in vitro. Several strains inhibited members of the genera Fusarium, Sclerotinia or Sclerotium and this
antagonism was related to their ability to produce secondary metabolites. A phylogenetic analysis based
on rpoB or 16S rRNA gene sequences confirmed that the isolates DGR22, MGR4 and MGR39 with high
biocontrol potential belonged to the genus