Magnetofection enhances adenoviral vector-based gene delivery in skeletal muscle cells

PEREYRA A; MYKHAYLYK O; FALOMIR LOCKHART E; TAYLOR J; DELBONO O; GOYA R; PLANK C; HEREÑÚ C

Journal of Nanomedicine and Nanotechnology

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Año: 2016 vol. 7 p. 364 - 364

bstract The goal of magnetic field-assisted gene transfer is to enhance internalization of exogenous nucleic acids by association with magnetic nanoparticles (MNPs). This technique named magnetofection is particularly useful in difficultto-transfect cells. It is well known that human, mouse, and rat skeletal muscle cells suffer a maturation-dependent loss of susceptibility to Recombinant Adenoviral vector (RAd) uptake. In postnatal, fully differentiated myofibers, the expression of the primary Coxsackie and Adenoviral membrane receptor (CAR) is severely downregulated representing a main hurdle for the use of these vectors in gene transfer/therapy. Here we demonstrate that assembling of Recombinant Adenoviral vectors with suitable iron oxide MNPs into magneto-adenovectors (RAd-MNP) and further exposure to a gradient magnetic field enables to efficiently overcome transduction resistance in skeletal muscle cells. Expression of Green Fluorescent Protein and Insulin-like Growth Factor 1 wa