The Cavβ1a Subunit Regulates Gene Expression and Suppresses Myogenin in Muscle Progenitor Cells

JACKSON TAYLOR; PEREYRA A; ZHANG T; MESSI ML; WANG ZM; HEREÑÚ C.; KUAN PE; DELBONO O

JOURNAL OF CELL BIOLOGY

ROCKEFELLER UNIV PRESS

Lugar: New York; Año: 2014 p. 829 - 829

0021-9525

he Cavβ1a Subunit Regulates Gene Expression and Suppresses Myogenin in Muscle Progenitor Cells. Voltage-gated calcium channel (Cav) β subunits are auxiliary subunits to Cav?s. Recent reports show Cavβ subunits may enter the nucleus, and suggest a role in transcriptional regulation, although the physiological relevance remains unclear. We sought to define the nuclear function of Cavβ in muscle progenitor cells (MPCs). We found that Cavβ1a is expressed in proliferating MPCs, before expression of calcium conducting subunit Cav1.1, and enters the nucleus. Loss of Cavβ1a expression impaired MPC expansion in vitro and in vivo, and caused widespread changes in global gene expression, including upregulation of myogenin. Additionally, we found that Cavβ1a localizes to the promoter region of a number of genes, preferentially at non-canonical (NC) E-box sites. Cavβ1a binds to a region of the Myog promoter containing an NC E-box, suggesting a mecha