Gene therapy for long-term restoration of circulating thymulin in thymectomized mice and rats.

(*IGUAL PARTICIPACIÓN) REGGIANI PC*, HEREÑÚ CB*; RIMOLDI OJ,; BROWN OA,; PLÉAU JM,; DARDENNE M,; GOYA RG.

GENE THERAPY

Nature Publishing group

Lugar: Londres; Año: 2006 vol. 13 p. 1214 - 1214

0969-7128

p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; mso-layout-grid-align: none">SUMMARY Thymulin is a bioactive thymic peptide involved in several aspects of intra- and extrathymic T-cell differentiation. We constructed a synthetic DNA sequence encoding met-FTS, a biologically active analog of thymulin, and subsequently cloned it into different expression vectors. A sequence optimized for expression of met-FTS in rodents, 5’-ATGCAAGCCAAATCTCAAGGTGGATCCAACTAGTAG-3’, was cloned in the mammalian expression vectors pCDNA3.1(+) and phMGFP (which expresses the Monster