Peripheral and mesencephalic transfer of a synthetic gene for the thymic peptide thymulin.

MOREL G; BROWN O; REGGIANI P; HEREÑÚ C.; PORTIANSKY E; ZUCCOLILLI G; PLEAU J; DARDENNE M; GOYA R

BRAIN RESEARCH BULLETIN

Elsevier

Lugar: Limmerik- Irlanda; Año: 2006 vol. 31 p. 647 - 647

0361-9230

p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 200%; TEXT-ALIGN: justify">Abstract- Morel et al 2006 Brain Research Bulletin Thymulin is a thymic peptide with antiinflammatory activity in the brain. We constructed a recombinant adenoviral vector, RAd-FTS, expressing a synthetic DNA sequence encoding met-FTS, a biologically active analog of thymulin and used it for peripheral and central gene transfer in rats. Thymulin concentration in serum and brain tissue was determined by bioassay. Reporter gene expression in the susbstantia nigra (SN) was quantitated by enzymohistochemistry or fluorescence microscopy using an appropriate image analysis software. A single intramuscular injection (10