IGF-1 Gene Transfer Modifies Inflammatory Environment and Gene Expression in the Caudate-Putamen of Aged Female Rat Brain

FALOMIR-LOCKHART, EUGENIA; DOLCETTI, FRANCO JUAN CRUZ; HERRERA, MACARENA LORENA; PENNINI, JERÓNIMO; ZAPPA VILLAR, MARÍA FLORENCIA; SALINAS, GABRIELA; PORTIANSKY, ENRIQUE; SPITTAU, BJÖRN; LACUNZA, EZEQUIEL; HEREÑÚ, CLAUDIA BEATRIZ #EQUAL CONTRIBUTION; BELLINI, MARÍA JOSÉ #EQUAL CONTRIBUTION

MOLECULAR NEUROBIOLOGY

HUMANA PRESS INC

Lugar: Oregon; Año: 2022

0893-7648

bstractBrain aging is characterized by chronic neuroinflammation caused by activation of glial cells, mainly microglia, leadingto alterations in homeostasis of the central nervous system. Microglial cells are constantly surveying their environment todetect and respond to diverse signals. During aging, microglia undergoes a process of senescence, characterized by loss oframifications, spheroid formation, and fragmented processes, among other abnormalities. Therefore, the study of changes inmicroglia during is of great relevance to understand age‐related declines in cognitive and motor function. We have targetedthe deleterious effects of aging by implementing IGF-1 gene transfer, employing recombinant adenoviral vectors (RAds) asa delivery system. In this study, we performed intracerebroventricular (ICV) RAd-IGF-1 or control injection on aged femalerats and evaluated its effect on caudate-putamen unit (CPu) gene expression and inflammatory state. Our results demonstratethat IGF-1