Preparation of protein nanoparticle by dynamic aggregation and ionizing-induced crosslinking

ACHILLI, ESTAFANIA; CASAJUS GONZALO; SIRI MACARENA; FLORES CONSTANZA; KADLUBOWSKI ; ALONSO SILVIA DEL V.; GRASSELLI MARIANO

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 486 p. 161 - 161

0927-7757

p id="spar0115">Globular proteins can be considered as nanotools useful to perform a variety of chemical reactions. These biological macromolecules have very complex and detailed topological characteristics responsible for their several functions, which can be used for technological applications. Albumin is one of the few commercially available proteins with potential use in the preparation of nanomaterials by the bottom-up strategy. Albumin nanoparticles (NPs) can be prepared by several methods, but the preservation of the structure of the native protein in the final structure has not yet received too much attention. Our research group has recently reported the use of ionizing radiation to obtain albumin NPs. The irradiation of an ethanol solution of bovine serum albumin (BSA) allows obtaining protein NPs in the size range of 20?40 nm. However, a plausible mechanism to explain the process of preparati