Radiolysis effect of the high proportion of ethanol in the preparation of albumin nanoparticle

SIRI MACARENA

RADIATION PHYSICS AND CHEMISTRY (OXFORD)

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2019

0969-806X

he effects of ionising radiation on proteins have been studied since the initial stages of this technology. These effects can be either direct or indirect, and many radical reactions are expected to occur according to the irradiation conditions and sample preparation. Recently, albumin nanoparticles have been prepared, for technological purposes, by irradiation of protein solutions in the presence of a high amount of ethanol.The radiation-induced crosslinking process in the protein nanoparticle preparation is a matter of study; therefore in the present work different experimental data and reported results are evaluated in order to discuss the radical reactions involved in this process.According to a quantitative analysis of solvent radiolysis, based on reported data and some key experimental determinations, the hydroxyethyl radicals and solvated electrons are the main radiolysis species present in the media. Selected free amino acids dissolved in water with a high amount of ethanol we