SIRI MACARENA
Capítulos de libros
Título:
Nanostructuring materials by ionizing radiation technology: Protein nanoparticle preparation and functionalization of track etched membranes
Autor/es:
GRASSELLI MARIANO; SOTO ESPINOZA SILVIA L.; MARTINEZ LEANDRO G.; CASAJUS GONZALO; SIRI MACARENA; ALONSO SILVIA DEL V.; SMOLKO E.E.; INTERNATIONAL ATOMIC ENERGY AGENCY
Libro:
Nanoscale Radiation Engineering of Advanced Materials for Potential Biomedical Applications
Editorial:
International Atomic Energy Agency
Referencias:
Lugar: Vienna; Año: 2015; p. 17 - 30
Resumen:
Radiation technology is an industrially available technology with a potential application in the field of material nanostructuring.
More than 20 years ago, it was demonstrated that it could be used for preparation of polymer microparticles with a very narrow
particle size distribution. The report shows how smaller particles, in the nanoscale range, can be prepared by γ ray irradiation of an
albumin solution containing a certain amount of ethanol. Particle sizes, between 10 nm and 20 nm can be tuned by changing the
solvent conditions and independent of the irradiation dose. Near CD, UV/VIS and fluorescence spectroscopies were used for protein
characterization in the nanoparticulate material. A scheme for a plausible mechanism of nanoparticle formation is also shown. Light
scattering data, under protein denaturing conditions, show the cross-linking effect by measuring a low increment in the hydrodynamic
radii of protein nanoparticles compared to the albumin under the same conditions. Track etched membranes are also nanostructured
materials developed from swift heavy ion bombardment. The report also demonstrates membrane functionalization by grafting
techniques. Two techniques for grafting polymerization ? by a simultaneous radiation induced technique and by a novel technique
using residual radicals ? have been compared. Fluorescence techniques were implemented in order to follow the grafting process
onto the tracks. The latter approach yields a modification that is homogeneously distributed in the nanochannels