Cyto- and in vivo toxicity of a bovine albumin nanoparticle

SIRI MACARENA; ALONSO SILVIA DEL V.

Mar del Plata

Congreso; LI Reunión SAIB 2015; 2015

Serum albumin is one of the most abundant proteins in the body. Its mainfunction is to be useful as a carrier for different substances. Our group managedto obtain a bovine serum albumin nanoparticle (BSA NP).The aim of this study is to characterise the BSA NP as a possible and efficientdrug delivery system. Specifically the aim was to study the NP?s toxicity indifferent study models. Human breast MCF-7 and prostate PC-3 carcinomacells were tested for their viability while incubated with the BSA NP with andwithout drug. Cell viability was determined by crystal violet, MTT and LDHassays after 4, 24 and 48 hours.Moreover, toxicity studies in in vivo Zebrafish model were carried out. The effectof each treatment was tested by well activity, viability, cardiotoxicity andmorphological malformations.Our results show that BSA NP is not toxic for tumoural cell lines whereas, BSANP with an antitumoural drug diminishes considerably the cell viability (c.a. 55%viability).As regards Zebrafish toxicity, BSA NP and BSA NP with the antitumoural drugproved to be equally toxic. Both diminished the larvae activity and generatedmorphological changes in the larvae tested.