Eudragit E100: antibiofilm and antivirulence properties allow propose this cationic polymer as an adjuvant to conventional antimicrobials in a promising approach against Pseudomonas aeruginosa infections

Cordoba

Congreso; Ricifa; 2021

Ricifa

Eudragit E100: antibiofilm and antivirulence properties allow propose this cationic polymer as an adjuvant to conventional antimicrobials in a promising approach against Pseudomonas aeruginosa infections.Ana Apas, Luciana Campagno, Melisa Corti, Fabiana Alovero Dpto. de Ciencias Farmacéuticas, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba and Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA) ? CONICET. Córdoba ? Argentina. aapas@unc.edu.ar (A.A) ; luciana.paula.campagno@unc.edu.ar (L.C.) ; melisacorti@gmail.com (M.C.) ; fabiana.alovero@unc.edu.ar* (F.A). Correspondence: aapas@unc.edu.ar (A.A) ; fabiana.alovero@unc.edu.ar (F.A.)?Presented at RICiFa 2021 (VI International Meeting of Pharmaceutical Sciences. Córdoba, Argentina. November 10-11-12/2021).Pseudomonas aeruginosa is an opportunistic pathogen that exhibits its pathogenesis property due to several factors and antimicrobial resistance is a substantial problem in its treatment. In this scenario, treatments targeting bacterial virulence have gained considerable interest. Eudragit E100® (Eu) is a cationic copolymer not fully characterized regarding antimicrobial properties. Previously, we demonstrated that Eu enhanced the efficacy of various antimicrobials against P.aeruginosa (planktonic) without exhibiting bactericidal action in itself. Here, we investigate the ability of Eu to prevent biofilm formation and inhibit the production of relevant virulence factors of multi-resistant P.aeruginosa. Eu concentration-dependent reduction in biofilm biomass was evidenced by crystal violet staining assay. Furthermore, a significant reduction in the production of pyocyanin, pyorubin and proteases was observed as well as in bacterial motility. Reduced pigmentation observed by extraction with chloroform/HCl as compared to untreated control was recovered after 48-72 h of incubation in polymer-free medium.Biofilm formation is a resistance mechanisms reported for P.eruginosa and virulence factors play an important role in the establishment and progression of infections. Therefore, the inhibition of biofilm in the initial stage as well as the reversible antivirulence properties exhibited by Eu allow justifying the use of this polymer as an adjuvant to conventional antimicrobials as a promising approach to prevent the spread of resistant infections by P.aeruginosa.Keywords: PSEUDOMONAS AERUGINOSA, PYOCYANIN, PYORUBIN, EUDRAGIT E100, BIOFILMS. FundingThis research was funded by a grant from SECyT - Universidad Nacional de Córdoba (Res N° 411/18). Conflicts of InterestThe authors declare no conflict of interest