Rhythms of glycerophospholipid synthesis in retinal inner nuclear layer cells.

GARBARINO PICO E; VALDEZ DJ; CONTÍN MA; PASQUARÉ SJ; CASTAGNET PI; GIUSTO NM; CAPUTTO BL; GUIDO ME

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2005 vol. 47 p. 260 - 260

p class="abstract">The present study demonstrates that the biosynthesis of phospholipids in the inner nuclear layer cells of the chicken retina displays daily rhythms under constant illumination conditions. The vertebrate retina contains circadian oscillators and photoreceptors (PRCs) that temporally regulate its own physiology and synchronize the whole organism to the daily environmental changes. We have previously reported that chicken photoreceptors and retinal ganglion cells (RGCs) present significant daily variations in their phospholipid biosynthesis under constant illumination conditions. Herein, we demonstrate that cell preparations highly enriched in inner nuclear layer cells also exhibit a circadian-regulated phospholipid labeling after the in vivo administration of [(32)P]phosphate or [(3)H]glycerol both in animals maintained under constant darkness or light for at least 48h. In constant darkness, there was a significant incorporation of both precursors into phospholipids w