MODIFICATION IN THE COMPONENTS BIODISTRIBUTION OF A VACCINE, USING AS A PLATFORM A LIQUID CRYSTAL NANOSTRUCTURE

MARIN, CONSTANZA; RUIZ MORENO, FEDERICO NAHUEL; MARIA FERNANDA SANCHEZ VALLECILLO; CHIODETTI, ANA L.; PALMA, SANTIAGO D.; MORÓN, GABRIEL; ALLEMANDI, DANIEL A.; PISTORESI-PALENCIA, MARÍA C.; MALETTO, BELKYS A.

Jornada; Jornadas de Posgrado CIBICI; 2018

In the last years much effort in vaccinology has focused on the new formulation strategies for subunit vaccines. We formulated OVA (antigen) and CpG-ODN with a nanostructure formed by self-assembly of 6-O-ascorbyl palmitate (Coa-ASC16). We showed that OVA/CpG-ODN/Coa-ASC16 (G2), elicited an adaptive immune response superior to those induced by an aqueous formulation, OVA/CpG-ODN (G1). However, we do not know exactly the mechanisms of action of Coa-ASC16. Hence, this work was focused on test the impact of this nanoformulation on biodistribution of vaccine components and early immune response.Methods: mice were s.c. immunized with G1 or G2. OVA and CpG-ODN were labeled with near-infrared fluorescent dye, and were measured with Odyssey® CLx at several time points. Cytokines/chemokines were evaluated in plasma by a multiplex assay at 1.5h p.i.Results: are indicated as G1 vs G2.Liver: OVA signal showed statistically significant differences, with G1>G2 at 20 min (p<0.05), 2 (p<0.0001) and 4h (p<0.0001) pi. Kidney: OVA signal showed significant differences with G1>G2 (p<0.05) at 20 min. No OVA signal was observed in spleen in any group.Injection site: OVA signal showed significant differences with G1G2 (p<0.001) and G1G2 (p<0.0001) and G1