Nitro-oleic acid, a ligand of CD36, reduces cholesterol accumulation by modulating oxidized-LDL uptake and cholesterol efflux in RAW264.7 macrophages

VAZQUEZ, MATIAS M.; GUTIERREZ, MARIA V.; SALVATORE, SONIA R.; PUIATTI, MARCELO; DATO, VIRGINIA ACTIS; CHIABRANDO, GUSTAVO A.; FREEMAN, BRUCE A.; SCHOPFER, FRANCISCO J.; BONACCI, GUSTAVO

Redox Biology

ELSEVIER SCIENCE INC

Año: 2020 vol. 36

2213-2317

acrophages play a pivotal role in the early stages of atherosclerosis development; they excessively accumulate cholesterol in the cytosol in response to modified Low Density Lipoprotein (mLDL). The mLDL are incorporated through scavenger receptors. CD36 is a high-affinity cell surface scavenger receptor that facilitates the binding and uptake of long-chain fatty acids and mLDL into the cell. Numerous structurally diverse ligands can initiate signaling responses through CD36 to regulate cell metabolism, migration, and angiogenesis. Nitro-fatty acids are endogenous electrophilic lipid mediators that react with and modulate the function of multiple enzymes and transcriptional regulatory proteins. These actions induce the expression of several anti-inflammatory and cytoprotective genes and limit pathologic responses in experimental models of atherosclerosis, cardiac ischemia/reperfusion, and inflammatory diseases. Pharmacological and genetic approaches were used to explore the actions of