Effect of NO2-OA on the oxidative stress and glial reactivity in Müller Glial Cells

VAGLIENTI MV; RIDANO ME; SUBIRADA P; PAZ MC; BARCELONA PF; BONACCI G; SANCHEZ MC

Vancouver

Congreso; ARVO-2019; 2019

Association for Research in Vision & Ophthalmology

Purpose: Inflammation, oxidative and nitrosative stress are involved in the pathogenesis of diabetic complications, including retinopathy. Metabolic changes that occur in Diabetes result in alterations of retinal blood barrier, which allows the extravasation of plasma proteins. α2-macroglobulin (α2M) is a proteinase inhibitor and a cargo protein of growth factors and cytokines in the blood and in the extracellular space. Previous work of our group has demonstrated that α2M induce glial reactivity in Müller Glial cells (MGCs) mediated by the increase of GFAP levels. Nitro-fatty acids (NO2-FA) are important electrophilic signaling mediators with anti-inflammatory and cytoprotective properties (Keap1/Nrf2 pathway). Our goal was to determine whether nitro-oleic acid (NO2-OA) could be beneficial for retinal cells against oxidative stress and glial reactivity in the human MGC line (MIO-M1).Conclusions: These results indicate that NO2-OA may act as an antioxidant protecting retinal cells from oxidative and α2M damage.