TLR9 activation is required for cytotoxic response elicited by baculovirus capsid display

MOLINARI, PAULA; CRESPO, MARÍA INÉS; MOLINA, GUIDO N.; DHO, NICOLÁS D.; MARINHO, FÁBIO V.; MALETTO, BELKYS; LECLERC, CLAUDE; OLIVEIRA, SERGIO C.; TABOGA, OSCAR; CEBRIAN, IGNACIO; MORÓN, GABRIEL

IMMUNOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2022

0019-2805

lthough the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) infects lepidopteran invertebrates as natural hosts, represents an efficient vector for vaccine development. Baculovirus surface display induces strong humoral responses against viruses and parasites. A novel strategy based on capsid display carrying foreign antigens in the AcMNPV particle further improved the immune response by eliciting CD8+ T cell activation. In this study, we analyze the intracellular mechanisms and signalling pathways involved in CD8+ T cell activation by capsid display. Our results show that baculovirus can attach to the cell surface, enter dendritic cells (DCs), transit within endocytic vesicles and escape to the cytosol for further degradation by the proteasome. We found that the availability of viral proteins, endosomal acidification, and proteasome activity are needed for efficient Major Histocompatibility Complex class-I presentation by baculovirus carrying Ovalbumin