Compositional domain immiscibility in whole myelin monolayers at the air-water interface and Langmuir-Blodgett films.

RAFAEL GUSTAVO OLIVEIRA; MAGGIO, B.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

ELSEVIER SCIENCE BV

Año: 2002 vol. 1561 p. 238 - 238

0005-2736

onomolecular layers of whole myelin membrane can be formed at the air^water interface from vesicles or from solvent solution of myelin. The films appear microheterogeneous as seen by epifluorescence and Brewster angle microscopy. The pattern consists mainly of two coexisting liquid phases over the whole compression isotherm. The liquid nature of the phases is apparent from the fluorescent probe behavior, domain mobility, deformability and boundary relaxation due to the line tension of the surface domains. The monolayers were transferred to alkylated glass and fluorescently labeled against myelin components. The immunolabeling of two major proteins of myelin (myelin basic protein, proteolipid-DM20) and of 2P,3P-cyclic nucleotide 3P-phosphodiesterase shows colocalization with probes partitioning preferentially in liquid-expanded lipid domains also containing ganglioside GM1. A different phase showing an enrichment in cholesterol, galactocerebroside and phosphatidylserine markers is also