Structural rearrangement of AgNP-OA

FERNÁNDEZ BORDÍN SANTIAGO P; VICO RAQUEL; GASPERINI-MALFATTI, ANTONIO; OLIVEIRA, RAFAEL GUSTAVO

Congreso; LI Reunión Anual de la Sociedad Argentina de Biofísica; 2023

Core-shell nanoparticles (NPs) are very particular nanomaterials due to their unique properties, the ability to induce effective biological responses, and easy functionalization. This versatility, added to biocompatibility and the fact that they can be studied through in vitro and in vivo assays, has made their growing implementation in biomedical sciences [1].In particular, silver nanoparticles (AgNPs) have attracted great attention for use as unconventional antimicrobial agents. Although there is currently still controversy around toxicity and biological behavior in vivo, AgNPs have been used for a long time as antimicrobial agents in the health industry, cosmetics, food storage, textile coatings, and within some environmental applications [2].In this work, hydrophobic AgNPs stabilized with a natural fatty acid, oleic acid (AgNP-OA), were synthesized, and their interactions with various biomembrane models were studied using Langmuir films. To achiEve this aim, we carried out grazing incidence at small angles X-ray scattering (GISAXS) measurements at the Brazilian Synchrotron Light Laboratory (LNLS). Through the analysis of the scattering intensity curves, we could infer characteristics of the structural organization of the sample. Then, We use computational simulations using the BornAgain software [3] to refine the structural parameters of the proposed model.References:[1] Lansdown A.B.G. (2010), Adv. Pharmacol. Sci. 16.[2] Villanueva, M. E., Lanterna, A. E., & Vico, R. V. (2019). Journal of colloid and interfacescience, 543, 247-255.[3] Pospelov, G., et al. (2020). Journal of Applied Crystallography, 53(1), 262-276.