Short transmembrane domains with high-volume exoplasmic halves determine retention of Type II membrane proteins in the Golgi complex

RODRIGO QUIROGA; ALEJANDRA TRENCHI; AYELEN GONZALEZ MONTORO; JAVIER VALDEZ TAUBAS; HUGO MACCIONI

COMPANY OF BIOLOGISTS LTD

Lugar: Cambridge; Año: 2013 p. 5344 - 5344

t is still unclear why some proteins that travel along the secretory pathway are retained in the Golgi complex whereas others make their way to the plasma membrane. Recent bioinformatic analyses on a large number of single-spanning membrane proteins support the hypothesis that specific features of the transmembrane domain (TMD) are relevant to the sorting of these proteins to particular organelles. Here we experimentally test this hypothesis for Golgi and plasma membrane proteins. Using the Golgi SNARE protein Sft1 and the plasma membrane SNARE protein Sso1 from Saccharomyces cerevisiae as model proteins, we modified the length of their TMDs and the volume of their exoplasmic hemi-TMD, and determined their subcellular localization both in yeast and mammalian cells. We found that short TMDs with high-volume exoplasmic hemi-TMDs confer Golgi membrane residence, whereas TMDs with low- volume exoplasmic hemi-TMDs, either short or long, confer plasma membrane residence to these proteins. R