Normally occurring human anti-GM1 immunoglobulin M antibodies and the immune response to bacteria

ALANIZ MARÍA E; LARDONE RICARDO D; YUDOWSKI SILVIA L; FARACE MARÍA I; NORES GUSTAVO A.

INFECTION AND IMMUNITY

The American Society for Microbiology

Lugar: Washington DC; Año: 2004 vol. 72 p. 2148 - 2148

0019-9567

p class="abstract">Anti-GM(1) antibodies of the immunoglobulin M (IgM) isotype are normal components of the antibody repertoire of adult human serum. Using a sensitive high-performance thin-layer chromatography (HPTLC) immunostaining assay, we found that these antibodies were absent in the umbilical vein and children <1 month of age but could be detected after 1 month of age. Although most of the children older than 6 months of age were positive, there were still a few negative children. The appearance of anti-GM(1) IgM antibodies showed a perfect concordance with two well-characterized antibacterial antibodies, anti-Forssman and anti-blood group A, which indicates a similar origin. We also studied IgM reactivity with lipopolysaccharides (LPSs) from gram-negative bacteria isolated from stool samples from healthy babies and from Escherichia coli HB101 in serum from individuals of different ages. We found a positive reaction with both LPSs in all the children more than 1 month of age