Perinatal Protein Restriction Impacts Nuclear O-GalNAc Glycosylation in Cells of Liver and Brain Structures of the Rat

GARAY, YOHANA C.; CEJAS, ROMINA B.; PERONDI, MARIA C.; GUTIERREZ, MARIA C.; PARODI, PEDRO; FERRERO, FRANCO A.; LARDONE, RICARDO D.; VALDOMERO, ANALIA ; CUADRA, GABRIEL R.; IRAZOQUI, FERNANDO J.

JOURNAL OF NUTRITION

AMER SOC NUTRITIONAL SCIENCE

Año: 2023

0022-3166

ackground: Post-translational modifications are key factors in the modulation of nuclear protein functions controlling cell physiology and an individual´s health.Objective: This study examined the influence of protein restriction during the perinatal period on the nuclear O-GalNAc glycosylation of cells from the liver and parts of the brain in the rat.Methods: Pregnant Wistar rats were divided into two groups on day 14 of pregnancy and fed ad libitum one of two isocaloric diets containing 24% (well-fed) or 8% (protein-restricted diet) casein until the end of experiment. Male pups were studied after weaning at 30 days of life. Animals and their organ/tissues (liver, cerebral cortex, cerebellum and hippocampus) were weighed. Cell nuclei were purified, and the presence in nucleus and cytoplasm of all factors required for the initiation of O-GalNAc glycan biosynthesis, i.e., the sugar donor (UDP-GalNAc), enzyme activity (ppGalNAc-transferase) and the glycosylation product (O-GalNAc glycan