ROMERO JORGE MIGUEL
Artículos
Título:
Extracellular S-nitrosoglutathione, but not S-nitrosocysteine or N2O3, mediates protein S-nitrosation in rat spinal cord slices
Autor/es:
ROMERO, JM AND BIZZOZERO, OA
Editorial:
Blackwell Science
Referencias:
Lugar: Oxford; Año: 2006 vol. 99 p. 1299 - 1299
Resumen:
p class="MsoNormal" style="margin: 0cm 0.9pt 0pt 0cm; text-align: justify;">There is evidence that protein S-nitrosothiols (PrSNOs) accumulate in inflammatory demyelinating disorders like multiple sclerosis and experimental allergic encephalomyelitis. However, very little is known regarding the mechanism by which PrSNOs are formed in target cells. The present study compares the ability of potential intercellular mediators of nitrosative damage including S-nitrosoglutathione (GSNO), S-nitrosocysteine and N2O3 to induce protein S-nitros(yl)ation in the spinal cord, a CNS region that is commonly affected in multiple sclerosis and experimental allergic encephalomyelitis. The results clearly demonstrate that while all three NO-donors cause S-nitrosation of proteins in cell-free systems, only GSNO is a viable S-nitrosating agent in rat spinal cord slices. Generation of PrSNOs with GSNO occurs by S-transnitrosation as the process was not inhibited