Genosensing applications of glassy carbon electrodes modified with multi-walled carbon nanotubes non-covalently functionalized with polyarginine

LÓPEZ MUJICA, MICHAEL

Micromachines

MDPI

Año: 2022

2072-666X

e report the advantages of glassy carbon electrodes (GCE) modified with multi-walled carbon nanotubes (MWCNTs) non-covalently functionalized with polyarginine (PolyArg) for the ad-sorption and electrooxidation of different DNAs and the analytical applications of the resulting platform. The presence of the carbon nanostructures, and mainly the charge of the PolyArg that supports them, facilitate the adsorption of the nucleic acids and produce a decrease in the over-voltages for guanine and adenine electrooxidation. As a proof-of-concept of possible GCE/MWCNTs-PolyArg biosensing applications, we develop an impedimetric genosensor for the quantification of microRNA-21 at femtomolar levels, using GCE/MWCNTs-PolyArg as platform for immobilizing the DNA probe, with successful application in enriched biological fluids.