Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples

FERNANDO GARAY; GREGGORY KISIEL; AIPING FANG; ERNO LINDNER

SPRINGER HEIDELBERG

Lugar: New York; Año: 2010 vol. 397 p. 1873 - 1873

p class="MsoNormal" style="text-align: justify; line-height: normal; margin: 0in 0in 0pt;">This article presents a simple chronoamperometric immunosensor for the quantitative assessment of creatine kinase MB (CK-MB) in 50 µL undiluted serum samples. The immunosensor consists of gold working and counter electrodes patterned onto a glass chip by thin-film photolithography and an external Ag|AgCl reference electrode. The detection limit (DL) of the chronoamperometric method is 13 ng/mL (DL = 2×RMSD/S, where RMSD is the residual mean standard deviation of the measured points around a calibration curve with a slope of S). In spiked serum samples, the response was linear up to 300 ng/mL