A robust and practically free of charge intermittent use glucose biosensor

COLOMBO, LUCAS; GARAY, FERNANDO

SENSORS AND ACTUATORS B-CHEMICAL

ELSEVIER SCIENCE SA

Año: 2017

0925-4005

n the past decades, great effort has been put in finding new electrode surface modifiers and enzyme immobilizer agents that prevent the enzyme leakage, minimize the effect of interfering species, retain the enzyme bioactivity, and enhance the sensor sensitivity. In this work, a sandwich-type glucose biosensor that keeps its sensitivity and operational linear range for more than a year is presented. After 5months of intermittent use, where the biosensor was exposed to more than 500 standard additions, it presented a limit of detection of 5μM, and the linear behavior was from 5μM to 3mM with a value of r2 =0.999. Besides, after 7months of its assembling, the biosensor was employed for assessing the glucose concentration of real serum samples and its performance was compared with the response of a commercial autoanalyzer. A year later, the biosensor still exhibited very good performance of its analytical parameters.The performance of identical sandwich-type biosensors is analyz