GARAY FERNANDO SEBASTIÁN
Congresos y reuniones científicas
Título:
Development of Multi Analyte Immunoassays for Cardiac Proteins
Autor/es:
E. LINDNER; Y. LIU; I-J CHEN; J. GUO; F. GARAY
Lugar:
Dobogoko, Hungary
Reunión:
Congreso; Matrafured 08; 2008
Resumen:
Towards the development of multi-analyte electrochemical imrnunoassays for the determination of cardiac proteins individually addressable microelectrode arrays (MEA) were integrated into passive pump-driven microfluidic devices. We report on (i) methods to describe the flow rate as function of time in a passive pump driven microfluidic systems. (ii) on the site-specific immobilization of different enzymes onto the individual MEA surfaces, (iii) the quantitative assessment of surface confined enzyme activities by measuring the products of the enzyme catalyzed reactions directly on gold MEA surfaces by chronoamperometry or by imaging the enzyme patterned microelectrode array surfaces by Scanning Electrochemical Microscopy (SECM), (iv) simulations of cyclic voltammograms to provide generally applicable guidelines for coplanar and recessed MEA design with optimal electrochemical properties.
