Giant vesicles, Laurdan, and two-photon fluorescence microscopy: evidence of lipid lateral separation in bilayers.

LUIS A. BAGATOLLI; S. SANCHEZ; T. HAZLETT; E. GRATTON

METHODS IN ENZYMOLOGY.

ELSEVIER ACADEMIC PRESS INC

Lugar: Burlington, MA, Estados Unidos; Año: 2003 vol. 360 p. 481 - 481

0076-6879

ur aim in this article is to elaborate on the use of fluorescence spectroscopytools in fluorescence microscopy. Advances in optical methods, optical components,acquisition electronics, and detectors have greatly enhanced our ability tocollect increasingly detailed spectroscopic data with the imaging optics of a lightmicroscope. The continued development of confocal microscopy (both one-photonand two-photon approaches), which has greatly increased the information availablethrough imaging, has allowed for rapid advances in fluorescence correlation spectroscopyand three-dimensional particle-tracking methods, both of which can nowbe performed in the microscope environment. At the present time, there are a numberof laboratories actively advancing this concept, that is, performing spectroscopyin a microscope, for a variety of protocols and generating exciting results in studiesranging from cell physiology to the mechanics of