Structural characterization and lipid composition of acquired cholesteatoma: a comparative study with normal skin.

M. BLOKSGAARD; V. SVANE-KNUDSEN; J.A. SØRENSEN; LUIS A. BAGATOLLI; J. BREWER

OTOLOGY & NEUROTOLOGY

LIPPINCOTT WILLIAMS & WILKINS

Lugar: Philadelphia; Año: 2012 vol. 33 p. 177 - 177

1531-7129

div class="">HYPOTHESIS: The goal of this work is to characterize the morphology and lipid composition of acquired cholesteatoma. We hypothesize that constitutive lipid membranes are present in the cholesteatoma and resemble those found in human skin stratum corneum.METHODS: We performed a comparative noninvasive structural and lipid compositional study of acquired cholesteatoma and control human skin using multiphoton excitation fluorescence microscopy-related techniques and high-performance thin-layer chromatography.RESULTS: The structural arrangement of the cholesteatoma is morphologically invariant along a depth of more than 200 μm and resembles the stratum corneum of hyperorthokeratotic skin. Lipid compositional analyses of the cholesteatoma show the presence of all major lipid classes found in normal skin stratum corneum (ceramides, long chain fatty acids, and cholesterol). Consistent with this, evaluation of Nile red and LAURDAN