Effect of detergents on the physicochemical properties of skin stratum corneum: a two-photon excitation fluorescence microscopy study.

M. BLOKSGAARD; J. BREWER; PASHKOVSKI E; ANANTHAPADMANABHAN KP; J.A. SØRENSEN; LUIS A. BAGATOLLI

International Journal of Cosmetic Science

Wiley

Año: 2014 vol. 36 p. 39 - 39

h4>OBJECTIVE: Understanding the structural and dynamical features of skin is critical for advancing innovation in personal care and drug discovery. Synthetic detergent mixtures used in commercially available body wash products are thought to be less aggressive towards the skin barrier when compared to conventional detergents. The aim of this work is to comparatively characterize the effect of a mild synthetic cleanser mixture (SCM) and sodium dodecyl sulphate (SDS) on the hydration state of the intercellular lipid matrix and on proton activity of excised skin stratum corneum (SC).METHOD: Experiments were performed using two-photon excitation fluorescence microscopy. Fluorescent images of fluorescence reporters sensitive to proton activity and hydration of SC were obtained in excised skin and examined in the presence and absence of SCM and SDS detergents.RESULTS: Hydration of the intercellular lipid matrix to a depth of 10 μm into the S