Imaging and modeling of acute pressure-induced changes of collagen and elastin microarchitectures in pig and human resistance arteries

M. BLOKSGAARD; T.M. LEURGANS; B. SPRONCK; M.H. HEUSINKVELD; B. THORSTED; K. ROSENSTAND; I. NISSEN; U.M. HANSEN; J. BREWER; L.A. BAGATOLLI; L.M. RASMUSSEN; A. IRMUKHAMEDOV; K.D. REESINK ; J.G.R. DE MEY

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY

AMER PHYSIOLOGICAL SOC

Lugar: Bethesda; Año: 2017

0363-6135

he impact of disease related changes in the extracellular matrix (ECM) on the mechanical properties of human resistance arteries largely remains to be established. Resistance arteries from both pig and human parietal pericardium (PRA) display a different ECM microarchitecture compared to frequently used rodent mesenteric arteries. We hypothesized that the biaxial mechanics of PRA mirror pressure induced changes in the ECM microarchitecture. This was tested using isolated pig PRA as model system, integrating vital imaging, pressure myography and mathematical modeling. Collagenase and elastase digestions were applied to evaluate the loadbearing roles of collagen and elastin, respectively. The incremental elastic modulus linearly related to the straightness of adventitial collagen fibers circumferentially and longitudinally (both R2≥0.99), while there was a nonlinear relationship to the internal elastic lamina elastin fiber branching angles. Mathematical modeling suggest