Effect of macromolecular crowding on the kinetics of glycolytic enzymes and the behaviour of glycolysis in yeast

THOKE, HENRIK S.; BAGATOLLI, LUIS A.; OLSEN, LARS F.

INTEGRATIVE BIOLOGY

ROYAL SOC CHEMISTRY

Año: 2018

1757-9694

ater is involved in all aspects of biological activity, both as a solvent and as a reactant. It is hypothesized that intracellular water is in a highly structured state due to the high concentrations of macromolecules inthe cell and that this may change the activity of intracellular enzymes due to altered binding affinities and allosteric regulations. Here we first investigate the kinetics of two glycolytic enzymes in artificially crowded aqueous solutions and show that crowding does indeed change their kinetics. Based on our kinetic measurements we propose a new model of oscillating glycolysis that instead of Michaelis?Mentenor Monod?Wyman?Changeux kinetics uses the Yang?Ling adsorption isotherm introduced by G. Ling inthe frame of the Association-Induction (AI) hypothesis. Using this model, we can reproduce previous experimental observations of the coupling of glycolytic oscillations and intracellular water dynamics, e.g., (i) during the metabolic oscillations, the latte