BAGATOLLI LUIS ALBERTO
Capítulos de libros
Título:
Membranes and Fluorescence microscopy
Autor/es:
LUIS A. BAGATOLLI; G. GEDDES
Libro:
Reviews in Fluorescence 2007
Editorial:
Springer
Referencias:
Año: 2009; p. 33 - 52
Resumen:
Fluorescence spectroscopy-based techniques using conventional fluorimeters
have been extensively applied since the late 1960s to study different aspects
of membrane-related phenomena, i.e., mainly relating to lipid-lipid and lipid-protein
(peptide) interactions. Even though fluorescence spectroscopy approaches provide
very valuable structurally and dynamically related information on membranes, they
generally produce mean parameters from data collected on bulk solutions of many
vesicles and lack direct information on the spatial organization at the level of single
membranes, a quality that can be provided by microscopy-related techniques.
In this chapter, I will attempt to summarize representative examples concerning
how microscopy (which provides information on membrane lateral organization
by direct visualization) and spectroscopy techniques (which provides information
about molecular interaction, order and microenvironment) can be combined to give a
powerful new approach to study membrane-related phenomena. Additionally along
this chapter, it will be discussed how membrane model systems can be further
utilized to gain information about particular membrane-related process like protein(
peptide)/membrane interactions.
have been extensively applied since the late 1960s to study different aspects
of membrane-related phenomena, i.e., mainly relating to lipid-lipid and lipid-protein
(peptide) interactions. Even though fluorescence spectroscopy approaches provide
very valuable structurally and dynamically related information on membranes, they
generally produce mean parameters from data collected on bulk solutions of many
vesicles and lack direct information on the spatial organization at the level of single
membranes, a quality that can be provided by microscopy-related techniques.
In this chapter, I will attempt to summarize representative examples concerning
how microscopy (which provides information on membrane lateral organization
by direct visualization) and spectroscopy techniques (which provides information
about molecular interaction, order and microenvironment) can be combined to give a
powerful new approach to study membrane-related phenomena. Additionally along
this chapter, it will be discussed how membrane model systems can be further
utilized to gain information about particular membrane-related process like protein(
peptide)/membrane interactions.
