BAGATOLLI LUIS ALBERTO
Capítulos de libros
Título:
LAURDAN fluorescence properties in membranes: a journey from the fluorometer to the microscope
Autor/es:
LUIS A. BAGATOLLI; Y. MÉLY; G. DUPORTAIL
Libro:
Fluorescent Methods to study Biological Membranes
Editorial:
Springer
Referencias:
Año: 2013; p. 3 - 36
Resumen:
After 32 years since its introduction, the particular fluorescence
properties of 6-lauroyl-2-(dimethylamino)-naphtalene (LAURDAN) in model
and biological membranes are revisited. This review includes a historical perspective
about the design, synthesis and initial description of the probe?s fluorescent
properties, a discussion about the proposed mechanism of LAURDAN
sensitivity to membrane lateral packing, and a detailed description of the definition
of the Generalized Polarization function. This article includes as well
examples of the different experimental strategies involving LAURDAN in
model and biological membranes, using both bulk fluorescence spectroscopy
measurements and spatially resolved information from fluorescence microscopy.
The value of this probe in the study of membrane structure and dynamics is
reflected in more than 330 papers reported in the existing literature.
properties of 6-lauroyl-2-(dimethylamino)-naphtalene (LAURDAN) in model
and biological membranes are revisited. This review includes a historical perspective
about the design, synthesis and initial description of the probe?s fluorescent
properties, a discussion about the proposed mechanism of LAURDAN
sensitivity to membrane lateral packing, and a detailed description of the definition
of the Generalized Polarization function. This article includes as well
examples of the different experimental strategies involving LAURDAN in
model and biological membranes, using both bulk fluorescence spectroscopy
measurements and spatially resolved information from fluorescence microscopy.
The value of this probe in the study of membrane structure and dynamics is
reflected in more than 330 papers reported in the existing literature.
