BAGATOLLI LUIS ALBERTO
Capítulos de libros
Título:
Monitoring membrane hydration with 2-(dimethylamino)-6-acylnaphtalenes fluorescent probes
Autor/es:
LUIS A. BAGATOLLI; E. DISALVO
Libro:
Membrane Hydration
Editorial:
Springer
Referencias:
Año: 2015; p. 105 - 125
Resumen:
A family of polarity sensitive fluorescent probes (2-(dimethylamino)-6-
acylnaphtalenes, i.e. LAURDAN, PRODAN, ACDAN) was introduced by Gregorio
Weber in 1979, with the aim to monitor solvent relaxation phenomena on protein
matrices. In the following years, however, PRODAN and particularly LAURDAN,
were used to study membrane lateral structure and associated dynamics. Once
incorporated into membranes, the (nanosecond) fluorescent decay of these probes
is strongly affected by changes in the local polarity and relaxation dynamics of
restricted water molecules existing at the membrane/water interface. For instance,
when glycerophospholipid containing membranes undertake a solid ordered (gel)
to liquid disordered phase transition the fluorescence emission maximum of these
probes shift 50 nm with a significant change in their fluorescence lifetime. Furthermore,
the fluorescence parameters of LAURDAN and PRODAN are exquisitely
sensitive to cholesterol effects, allowing interpretations that correlate changes in
membrane packing with membrane hydration. Different membrane model systems
as well as innate biological membranes have been studied with this family of probes
allowing interesting comparative studies. This chapter presents a short historical
overview about these fluorescent reporters, discusses on different models proposed
to explain their sensitivity to membrane hydration, and includes relevant examples
from experiments performed in artificial and biological membranes.
acylnaphtalenes, i.e. LAURDAN, PRODAN, ACDAN) was introduced by Gregorio
Weber in 1979, with the aim to monitor solvent relaxation phenomena on protein
matrices. In the following years, however, PRODAN and particularly LAURDAN,
were used to study membrane lateral structure and associated dynamics. Once
incorporated into membranes, the (nanosecond) fluorescent decay of these probes
is strongly affected by changes in the local polarity and relaxation dynamics of
restricted water molecules existing at the membrane/water interface. For instance,
when glycerophospholipid containing membranes undertake a solid ordered (gel)
to liquid disordered phase transition the fluorescence emission maximum of these
probes shift 50 nm with a significant change in their fluorescence lifetime. Furthermore,
the fluorescence parameters of LAURDAN and PRODAN are exquisitely
sensitive to cholesterol effects, allowing interpretations that correlate changes in
membrane packing with membrane hydration. Different membrane model systems
as well as innate biological membranes have been studied with this family of probes
allowing interesting comparative studies. This chapter presents a short historical
overview about these fluorescent reporters, discusses on different models proposed
to explain their sensitivity to membrane hydration, and includes relevant examples
from experiments performed in artificial and biological membranes.
